This research is on carbon flux and the differences in sterile soil and non sterile. The point of this research was to figure out if the sterile soil had an effect on carbon output and if the bacteria and fungi have an effect on the carbon flux. We wanted to know if the sterile soil has a effect on the photosynthesis of trees. To find out whether this is true or not we took fourteen different tubes and analyzed them with a few different tests. Three tubes where no bacteria no tree, bacteria and tee, sterile soil no tree, sterile soil and tree and two tubes of vermiculite. The first thing we did with the tubes was testing the carbon fluxes. We used the Li-Core 6400 to measure the carbon flux and photosynthesis of the tree. Another one of the tests was growing Bacteria and fungi. We took 50g soil from each tube and put it into 450ml of water. Once we combined the soil and the water we had to vortex it for four minutes. After that was done we took 1ml of that water and put it into test tubes of 9ml of water with pipettes. We put the water on the agar plates so that our bacteria grew. By keeping count of the bacteria this tells us how dense the soil was and how many microbes are in it. In the end we learned that sterile soil doesn’t have an effect on the photosynthesis of the trees. We also learned that the bacteria and fungi do have an effect on the output of carbon.